I’m like 95% sure I’ve seen that somewhere around Orlando, and the sign looks like a Turnpike sign, but the turnpike doesn’t go to Tampa unless you go turnpike to I-4…
EDIT: there’s a less crunchy image on Reddit, apparently FDOT posted it to Twitter. That is definitely a Florida Turnpike sign (sorry for linking to Reddit)
EDIT 2: Found the original tweet via wayback machine, which was deleted by FDOT (gee, I wonder why?) and it’s somewhere in Palm Beach County (Lyons Road to the Turnpike), not Orlando.